安徽医科大学学报2012，Vol.47Issue(4)：375-378,4.
氧化应激促进凋亡相关因子在糖尿病大鼠坐骨神经中表达的研究
Study on oxidative stress promoting the expression ofapoptosis- associated factors in sciatic nerve of diabetic rat
摘要
Abstract
To investigate oxidative stress injury with the expression and correlativity of apoptosis-associated factors in sciatic nerve of diabetic rat in early stage, and further discuss the pathogenesis of diabetic peripheral neuropathy ( DPN ). Methods A single intra-peritoneal injection of streptozotocin( STZ ) was used to induce diabetic rats models,and 17 diabetic rats were picked up randomly as diabetic group ( M group ). Normal control group ( N group )was set up too. 8 weeks later, left sciatic nerves were dissected, and morphological alteration was valued by HE staining. The expression of 8-hydroxy-2-deoxyguanosine ( 8-OHdG ) and Caspase-3 in sciatic nerve tissue was detected by immunohistochemistry, and the expression of dynamin-related protein 1 ( Drp-1 ) and Bax was detected by western blot. Results Compared with N group, sciatic nerve tissues in M group showed obvious morphological alteration, the body weight of rats did not increase significantly, but blood glucose levels raised significantly ( P <0. 05 ),and the expression of protein Drp-1, Bax, 8-OHdG and Caspase-3 also increased significantly( P < 0. 05 ). In M group, the expression of 8-OHdG showed a positive correlation with Caspase-3( r = 0. 798, P < 0. 05 ). Conclusion Oxidative stress in sciatic nerve of early diabetic rats induces overexpression of apoptosis-asso-ciated factors, and finally promotes apoptosis may play an important role in contributing DPN.关键词
糖尿病大鼠/坐骨神经/Drp-1/8-OHdG/凋亡相关因子Key words
diabetic rat/ sciatic nerve/ drp-1/ 8-OHdG/ apoptosis-associated factors分类
医药卫生引用本文复制引用
施丽丽,任明山,吴元洁,吴元波,沈玲玲..氧化应激促进凋亡相关因子在糖尿病大鼠坐骨神经中表达的研究[J].安徽医科大学学报,2012,47(4):375-378,4.基金项目
国家自然科学基金(编号:81102626) （编号:81102626）
