安徽医科大学学报2012，Vol.47Issue(4)：396-399,4.
rAd-p53对人肝癌细胞放射敏感性影响的实验研究
Experimental study of the effects of rAd-p53 on theradiosensitivity of human hepatocellular carcionoma
摘要
Abstract
To observe the effect and difference of rAd-p53 on the radiosensitivity for human hepatocellular carcionoma( HCC ). Methods Two HCC cell lines with different p53 genetic statuses, PLC/PRF/5( MT p53 ) and HepG2( WT p53 ) were infected with recombinant adenovirus carrying wild-type( WT ) p53 gene( rAd-p53 ) and Ad-EGFP, and cell survival was evaluated by MTT; Radiosensitivity was evaluated by clonogenic assay. Cell apoptosis was assayed by flow cytometry. Results 48 h after infection efficiency of 15MOI values of rAd-p53 to PLC/PRF/5 and HepG2 cells, and cell survival rates of PLC/PRF/5 and HepG2 cells were( 7. 30 ± 1. 78 )% and ( 11.51 ±1. 53 )%. The transduction efficiency was gradually increased with the infection time or virus dosage in-creased( P <0. 01 ). SER value of HepG2 and PLC/PRF/5 were 1. 24 and 1. 52 by clonogenic assay,cell apoptosis was( 22. 19 ± 1. 04 )% and( 30. 36 ±2. 74 )% respectively and the effects of rAd-p53 group was stronger than oth-ers( P <0. 01 ). Conclusion rAd-p53 can significantly inhibit proliferation, induce cell apoptosis and increase radiosensitivity of HCC, but the aforesaid efficacy of rAd-p53 in PLC/PRF/5 cells( MT p53 ) is stronger than that in HepG2 cells( WT P53 ).关键词
肝癌/重组人p53腺病毒/放射治疗/敏感性Key words
hepatocellular carcinoma/ recombinant human adenovirus p53 / radiotherapy分类
医药卫生引用本文复制引用
陶振超,邱俊,钱立庭,王明明,吴爱东,闫冰..rAd-p53对人肝癌细胞放射敏感性影响的实验研究[J].安徽医科大学学报,2012,47(4):396-399,4.基金项目
安徽省卫生厅医学科研重点项目(编号:2010A004) （编号:2010A004）
