安徽中医学院学报2012，Vol.31Issue(2)：44-47,4.DOI:10.3969/j.issn.1000-2219.2012.02.015
新藤黄酸与细胞外信号调节激酶抑制剂PD98059联合应用对肺腺癌A549细胞生长的影响
Effects of Gambogenic Acid Combined with Extracellular Signal Regulated Kinase Specific Inhibitor PD98059 on Growth of Lung Adenocarcinoma A549 Cells
摘要
Abstract
Objective To investigate the effects of Gambogenic acid (GNA) combined with extracellular signal regulated kinase (ERK) specific inhibitor PD98059 on the growth of pulmonary adenocarcinoma A549 cells. Methods A549 cells were treated with GNA and PD98059. Morphologic changes of A549 cells were detected by inverted microscope. Cell viability was measured by methyl thiazolyl tetrazolium (MTT)staining. The changes of intracellular reactive oxygen species (ROS) were determined by flow cytometry,and the expressions of ERK and P-ERK proteins were detected by western blotting method. Results MTT staining results showed that 2. 5 μmol/L GNA significantly inhibited the proliferation of A549 cells, its inhibitive effects were obviously enhanced in combination with PD98059 for 24 h. After treated with GNA for 24 h, inverted microscope showed that A549 cells became pyknotic, round, and smaller, some A549 cells were in a semi-adherence state. After treated with GNA combined with PD98059 for 24 h, most A549 cells became pyknotic and round, then dropped out and suspended in the medium; a few A549 cells became larger, swelled, ruptured, and became into necrosis. Flow cytometry showed that the production of ROS in A549 cells treated with 2. 5 μmol/L GNA was significantly more than that in A549 cells treated with 20 μmol/L PD98059 plus 2. 5 μmol/L GNA for 24 h. Western blotting detection showed that the expression levels of p-ERK protein were significantly decreased in A549 cells treated with PD98059 plus GNA in comparison with those in A549 cells treated with GNA for 24 h. Conclusion GNA can induce apoptosis of pulmonary adenocarcinoma A549 cells. The combined use of ERK specific inhibitor PD98059 and GNA can reduce the activation of ERK protein, and increase the apoptosis of A549 cells. The preliminary results indicated that the GNA-induced apotosis of A549 cells may be related to regulating ERK pathway.关键词
新藤黄酸/细胞外信号调节激酶抑制剂/A549细胞/细胞凋亡Key words
Gambogenic acid / extracellular signal regulated kinase specific inhibitor/ A549 cells/ cell apoptosis分类
医药卫生引用本文复制引用
彭程,苏婧婧,程卉,李庆林..新藤黄酸与细胞外信号调节激酶抑制剂PD98059联合应用对肺腺癌A549细胞生长的影响[J].安徽中医学院学报,2012,31(2):44-47,4.基金项目
国家自然科学基金项目(81173600) （81173600）
安徽省自然科学基金项目(11040606M190) （11040606M190）
