北华大学学报：自然科学版2011，Vol.12Issue(5)：553-555,3.
染色质免疫沉淀技术（ChIP）在基因转录调控中的作用
The Role of Chromatin Immunoprecipition（ChIP） in Gene Transcriptional Regulation
史双林1
作者信息
- 1. 北华大学医学检验学院,吉林吉林132013
- 折叠
摘要
Abstract
Objective To detect the interaction of transcription factor in vivo and DNA（regions of the target gene promoters）.Method The cells were fixed with Formaldehyde,the protein-DNA complex was cross-linked under the physiologic condition,DNA was broken into small fragments with sonication,the immunoprecipitation for the complex was performed with specific antibodies and heated at 65℃ to reverse the formaldehyde cross-linking,and DNA was purified by PCR.Results The fragments were amplified by using PCR to show positive bands.Conclusion ChIP is a powerful technique that endogenous transcription factors is combined DNA under different physiologic conditions.关键词
ChIP/转录因子/DNA/启动子Key words
Chromatin immunoprecipition/transcription factor/DNA/promoter分类
基础医学引用本文复制引用
史双林..染色质免疫沉淀技术（ChIP）在基因转录调控中的作用[J].北华大学学报：自然科学版,2011,12(5):553-555,3.
