北京林业大学学报2011，Vol.33Issue(5)：97-102,6.
拟南芥HsfA2基因转化地被菊的研究
Transformation of ground-cover Chrysanthemum with HsfA2 gene isolated from Arabidopsis thaliana
摘要
Abstract
A HsfA2 gene isolated from Arabidopsis thaliana ecotype Columbia was transferred into ground-cover Chrysanthemum ＇Beilinhuang＇ by the Agrobacterium-mediated method.After antibiotics screening and molecular detection,eight positive transgenic lines were obtained.RT-PCR detection results showed that the exogenous AtHsfA2 gene was expressed in seven transgenic lines.The results of heat stress treatment also showed that the ability of transgenic plants to heat stress was significantly higher than that of non-transgenic plants.This research obtained Chrysanthemum lines with higher resistance ability to heat stress through transgenic method and may provide scientific references for transgenic breeding of ornamental plants.关键词
AtHsfA2基因/遗传转化/地被菊/热胁迫Key words
AtHsfA2 gene/genetic transformation/ground-cover Chrysanthemum/heat stress分类
农业科技引用本文复制引用
赵伶俐,石少川,孙佳琦,张启翔,高亦珂..拟南芥HsfA2基因转化地被菊的研究[J].北京林业大学学报,2011,33(5):97-102,6.基金项目
“863”国家高技术研究发展计划项目 （）
