北京林业大学学报2011，Vol.33Issue(6)：90-93,4.
ipt基因促进杜仲遗传转化不定芽再生
Ipt gene promoting shoot regeneration in genetic transformation of Eucommia ulmoides Oliv
摘要
Abstract
In this research,the ipt gene driven by 35S promoter was transferred into Eucommia ulmoides Oliv.via agrobacterium-mediated transformation,and the influence of exogenous gene on shoot regeneration was further investigated in the current work.The results showed that compared with control（ipt-free） line（0.93%）,no significant difference in shoot regeneration efficiency was obtained from the ipt transgenic lines（0.96%）.However,ipt gene enhanced the shoot number per explant,which was 3.1,obviously higher than that of control（1.9）.Thus ipt gene enhanced shoot inductivity of E.ulmoides,which was 2.95%,evidently high than CK 1.68%.The present research firstly prove that ipt gene can substantially enhance shoot regeneration of E.ulmoides and this lays a foundations for promoting genetic transformation efficiency of E.ulmoides.关键词
异戊烯基转移酶基因/遗传转化/杜仲Key words
ipt gene/genetic transformation/Eucommia ulmoides Oliv分类
农业科技引用本文复制引用
李岩,赵德刚..ipt基因促进杜仲遗传转化不定芽再生[J].北京林业大学学报,2011,33(6):90-93,4.基金项目
国家转基因新品种培育重大专项 （）
贵州省重大专项（黔科合重大专项字（2007）6004-6） （）
