北京林业大学学报2011，Vol.33Issue(6)：186-190,5.
大花红景天悬浮颗粒、悬浮细胞体系的研究
Callus particles and cell suspension cultures of Rhodiola crenulata
摘要
Abstract
To investigate the relations between salidroside content and particle size as well as growth conditions,also the possible mechanism of Rhodiola crenulata,callus obtained from leaves of R.crenulata was used for raising particle suspension cultures,which were then filtered by cell sieves with 60,80,100 or 120 mesh respectively,so that cell suspension cultures were established.The salidroside content in particle suspension cultures（the average diameter was about 2.5 cm） and cell suspension cultures（filtered by 80 mesh cell sieve） were detected,both of the particle and cell culture solutions in the same culture conditions were also detected.After being filtered by 80 mesh cell sieve and then cultured for a period of time,the culture filtrate had been shown to produce vast rapid proliferated cell suspension cultures with uniform size.Cell suspension cultures had 0.606 mg/g of salidroside,which was about 2.2 times of that of particle suspension cultures（0.27 mg/g）.However,neither of the solution of particle suspension cultures nor cell suspension cultures was found to have salidroside.It is concluded that salidroside content in suspension cultures has certain relations with particle size and growth condition of different cell aggregation.Since small cells contact fully with oxygen and nutrient of medium,they can produce high content of salidroside.关键词
大花红景天/悬浮颗粒/悬浮细胞/高效液相色谱/红景天苷Key words
Rhodiola crenulata/particle suspention cultures/cell suspension cultures/high performance liquid chromatography（HPLC）/salidroside分类
农业科技引用本文复制引用
王彩云,丁友芳,翁慧,史玲玲,闫林林,刘玉军..大花红景天悬浮颗粒、悬浮细胞体系的研究[J].北京林业大学学报,2011,33(6):186-190,5.基金项目
“十一五”国家科技支撑计划重大专项 （）
