北京林业大学学报2012，Vol.34Issue(1)：80-85,6.
多杀菌素对舞毒蛾保护酶及几丁质酶的影响
Effects of spinosad on the activity of protective enzymes and chitinase in Lymantria dispar
摘要
Abstract
In order to better understand the insecticidal activity and mode of action of spinosad,its effect on the activity of protective enzymes and chitinase of 3rd and 5th instar larvae of Lymantria dispar was assayed in this study.The results showed that spinosad had no effect on catalase（CAT） activity,but significantly changed phenoloxidase（PO） activity in an activation-inhibition fashion for both 3rd and 5th instar larvae.The same significant ＂activation-inhibition＂ effect was also found on the activity of superoxide dismutase（SOD） and peroxidase（POD） in the 3rd instar larvae（P0.01）,with the maximum enzyme activations occurred 3-6 hours after spinosad treatment.Whereas the POD and SOD activity in the 5th instar larvae was significantly increased within 3 hours after the treatment（P0.01;with treatment/CK ratios being 1.543 and 1.716,respectively）,and no significant treatment effects were detected after 3-6 hours.The chitinase activity in the 3rd instar larvae was significantly activated 3-12 hours after spinosad treatment,while it was strongly inhibited in the 5th instar larvae 12-24 hours after the treatment.In conclusion,spinosad shows a significant effect on the activity of most protective enzymes and chitinase,and can interrupt normal metabolism of L.dispar larvae,therefore,has a potential to be an effective pesticide against this serious defoliator pest.关键词
多杀菌素/舞毒蛾/保护酶/几丁质酶Key words
spinosad/Lymantria dispar/protective enzymes/chitinase分类
农业科技引用本文复制引用
鄢杰明,严善春,曹传旺..多杀菌素对舞毒蛾保护酶及几丁质酶的影响[J].北京林业大学学报,2012,34(1):80-85,6.基金项目
林业公益性行业科研专项 （）
东北林业大学研究生创新基金项目 （）
