北京师范大学学报：自然科学版2011，Vol.47Issue(4)：393-397,5.
抗栓胰岛素原钙调素重组融合蛋白表达条件的摸索
EXPLORATION OF AN ANTITHROMBOTIC PROINSULIN-CAMODULIN FUSION PROTEIN
摘要
Abstract
Chimeric antithrombotic proinsulin mutant （Mr = 7.0 ×10^3） was constructed based on inactive proinsulin scaffold with GP Ⅱ b Ⅲa receptor antagonist disintegrin sequence replacing connecting peptide of proinsulin and was further fused with camodulin at C-terminal region. The fusion protein （Mr = 24. 7 ×10^） was expressed at high-level in an E. coli BL21（DE3） host-cell system. Variations in temperature, culture medium, and induction method were considered for optimal expression.关键词
抗栓胰岛素原/钙调素/融合蛋白/表达Key words
antithrombosis proinsulin/camodulin/fusion protein/expression分类
生物学引用本文复制引用
井健..抗栓胰岛素原钙调素重组融合蛋白表达条件的摸索[J].北京师范大学学报：自然科学版,2011,47(4):393-397,5.基金项目
北京师范大学自主科研基金资助项目 （）
