北京师范大学学报：自然科学版2011，Vol.47Issue(4)：398-404,7.
等边浅蛤（Gomphina aequilatera）核糖体DNA第一内转录间隔区序列的特征分析
CHARACTERIZATION OF INTERNAL TRANSCRIBED SPACER ONE IN GOMPHINA AEQUILATERA RIBOSOMAL DNA
摘要
Abstract
Internal transcribed spacer one （ITS-1） in ribosomal DNA is a of the molecular marker frequently used in phylogeny, population genetics and species taxonomy studies. However, the presence of variations among multiple copies within individual genome is often neglected. In this study, Gomphina aequilatera ITS-1 sequences of two individual subjects from six sampling sites each at intertidal zone along Zhejiang and Fujian provinces were obtained by polymerase chain reaction, molecular cloning and sequencing. Both an 17 bp segment of insertion/deletion and an imperfect microsatellite DNA with structure of （GA）3 （GGGA）2 （GA）4-6 were detected by sequence alignment G. aequilatera ITS-1 sequence length variations intragenome or among different individuals were caused mainly by those two structures. Statistical analysis revealed that G. aequilatera ITS-1 sequence variation intra-genome was not significantly different from sequence variation between individuals in the same sampling site （P 〉 0.05 ）, but was significantly different from sequence variation among individuals at different sampling sites （P 〈0.05）. Such sequence variation is therefore an effective marker at population level. Phylogeny reconstruction and network analysis indicated that G. aequilatera ITS-1 haplotypes were closely related..关键词
ITS-1/分子标记/序列变异/等边浅蛤Key words
ITS-1/molecular marker/sequence variation/Gomphina aequilatera分类
生物学引用本文复制引用
吕锋骅,韩洁,林岿璇,陈万东..等边浅蛤（Gomphina aequilatera）核糖体DNA第一内转录间隔区序列的特征分析[J].北京师范大学学报：自然科学版,2011,47(4):398-404,7.基金项目
北京师范大学优秀博士学位论文培育基金资助项目 （）
浙江省温州市科技局研究资助项目 （）
