北京大学学报（医学版）2012，Vol.44Issue(1)：39-42,4.DOI:10.3969/j.issn.1671-167X.2012.01.008
溶胶-凝胶生物活性玻璃对人牙髓细胞作用的研究
Effects of ionic-dissolution of sol-gel bioactive glasses on human dental pulp cells
摘要
Abstract
Objective:To evaluate the effects of ionic-dissolution of sol-gel bioactive glasses (BG) on proliferation and alkaline phosphatase (ALP) activity of human dental pulp cells (HDPCs) . Methods; The primary HDPCs were isolated from intact premolar and third molars and were cultured in DMEM. Then the 4 th generation of HDPCs were cultured in DMEM, which contained 1 g/L of one of 58S, Nano-58S, and 45S5 ionic-dissolution products. Meanwhile HDPCs were cultured in DMEM without BG as control group. Proliferation of the cells was evaluated by MTT assay on days 1,3,5 and 7. ALP activity was measured on day 14 using ALP Assay Kit. Results; Compared with the control group, HDPCs' proliferation in the Nano-58S group increased significantly. HDPCs cultured in Nano-58S and the 58S groups showed higher ALP activity (P <0.01) ; but in 45S5 group showed an inhibition on the HDPCs' proliferation (P<0. 01) and lower ALP activity (P < 0. 05). Conclusion: Present work has shown that Nano-58S and 58S sol-gel BG can promote the proliferation and ALP activity of HDPCs.关键词
牙髓/细胞增殖/碱性磷酸酶/生物相容性材料/细胞,培养的Key words
Dental pulp/ Cell proliferation/ Alkaline phosphatase/ Biocompatible materials/ Cells,cultured分类
医药卫生引用本文复制引用
米姗姗,董艳梅,高学军..溶胶-凝胶生物活性玻璃对人牙髓细胞作用的研究[J].北京大学学报（医学版）,2012,44(1):39-42,4.基金项目
国家自然科学基金项目资助(50830101) （50830101）
