北京中医药大学学报2011，Vol.34Issue(12)：836-842,7.
三七皂苷在大鼠外翻肠囊中的吸收及与P-糖蛋白相互作用研究
Absorption of panax notoginseng saponins in rat everted gut sac and interaction between it and P-glycoprotein
摘要
Abstract
Objective To study the absorption characteristics of effective components of panax notoginseng saponins (PNS) including ginsenoside Rg1, ginsenoside Rb1 and notoginsenoside R1 in rat model of everted gut sac and interaction between PNS and P-glycoprotein ( P-gp). Methods A quantitative determination was established for detecting ginsenoside Rg1, ginsenoside Rbj and notoginsenoside R1 by using HPLC. The influences of different time points, concentration and transport directions on the absorption and transportation of ginsenoside Rg1, ginsenoside Rb, and notoginsenosideR, in rat small intestine by applying rat model of everted gut sac. The changes of absorptive amount of ginsenoside Rg,, ginsenoside Rb, and notoginsenoside R, were observed before and after combined use of PNS effective components and verapail ( Ver) , an inhibitor of P-gp. The blank group, Ver positive control group ( Ver group) , high-dose, mid-dose and low-dose effective components of PNS group ( high-dose PNS group, mid-dose PNS group and low-dose PNS group) were set up, and the influence of effective components of PNS on the transportation of Rhol23 ( substrate of P-gp) was observed. Results The absorption and transportation of ginsenoside Rg,, ginsenoside Rb1 and notoginsenoside R, in rat small intestine were all linear absorption in different time points and concentration and all regression coefficients (r) were over 0. 9, which accorded with grade 0 absorption rate. The absorption and transportation of PNS effective components were significantly influenced by transport directions, and the transport amount from serosal surface to mucosal surface (B-A) was much higher than that from mucosal surface to serosal surface ( A-B). After PNS effective components combined with Ver, the absorption amount of ginsenoside Rgi, ginsenoside Rb, and notoginsenoside R, in rat small intestine increased significantly (P <0.05). PNS effective components promoted the transport amount of Rhol23 and it was significantly different compared with the blank group ( P < 0. 05 ). This efficiency was especially significant in the high-dose PNS group and the difference was greatly significant compared with Ver group (P<0. 01). Conclusion The absorption and transportation of PNS effective components-ginsenoside Rg,, ginsenoside Rb, and notoginsenoside R, in rat small intestine are all linear absorption, so the absorption mechanism can be thought as passive transport mechanism with obvious feature of P-gp substrate transportation and inhibition of P-gp substrate exocytosis.关键词
三七皂苷/外翻肠囊/P-糖蛋白/高效液相色谱/大鼠Key words
panax notoginseng saponins/ everted gut sac/ P-glycoprotein/ HPLC/ rats分类
医药卫生引用本文复制引用
萨础拉,吕航,姜艳艳,巴寅颖,石任兵..三七皂苷在大鼠外翻肠囊中的吸收及与P-糖蛋白相互作用研究[J].北京中医药大学学报,2011,34(12):836-842,7.基金项目
高等学校科技创新工程重大项目培育资金资助项目(No.706008),北京中医药大学自主选题项目(No.JYBZZ-XS038) （No.706008）
