草业科学2011,Vol.28Issue(9):1685-1689,5.
洗涤剂对豌豆根尖细胞的致畸效应
Effect of detergent concentrations on root tip cells teratogenesis of pea
摘要
Abstract
the micronucleus test was used to determine the effects of detergent concentrations on root tip cells teratogenesis of pea by measuring the cytotoxicity and the genotoxicity. In this study, the dilution detergent concentrations with 200 volume (detergent/distilled water), 100 volume, 10 volume and 5 volume, respectively, was added into pea root tip and root point cell mitosis index, the micronucleus rate and chro- mosomic aberration rate were investigated and made micrography at 24 h and 48 h. The results of this study showed that the root tip cell mitosis index obvlously decreased and had the obvious dosage-time effectiveness, and the micronucleus rate and the chromosomic aberration rate increased in low concentrations, but they decreased in high concentrations and they did not have the obvious dosage-time effect along with the increase of detergent diluted concentration and with the lengthens of treatment time. The cell chromosome bridge, micronucleus and many kinds of chromosomic aberration type were caused with different concentrations detergent, and the root tip cell chromosome damage was created as well. This study suggested that the different concentrations detergent had some certain heredity toxicity to the pea root tip cell.关键词
洗涤荆/豌豆根尖细胞/有丝分裂指数/微核率/染色体畸变率Key words
detergent/pea root tip cell/mitotic index/micronucleus rate/chromosomic aberration rate分类
农业科技引用本文复制引用
曾秀存,许耀照,王勤礼,李彩霞..洗涤剂对豌豆根尖细胞的致畸效应[J].草业科学,2011,28(9):1685-1689,5.基金项目
河西学院教学研究课题 ()