草业科学2011,Vol.28Issue(11):1930-1935,6.
野牛草种质基于SRAP标记的遗传多样性研究
Genetic diversity of Buchloe dactyloides based on SRAP markers
摘要
Abstract
Buchloe dactyloides is a warm-season grass for low-maintenance lawns. In this paper, sequencerelated amplified polymorphism (SRAP) molecular markers were used to detect the genetic diversity of 31 accessions of B. dactyloides collected from America, Canada and Japan. Nineteen primer pairs were used to amplify among the accessions. A total of 271 (86.86%) bands were observed. The genetic diversity coefficients (GS) varied from 0. 586 5 to 0. 916 7 among these accessions, with an average of 0. 669 1. Two materials showed the specific and specific deletion bands respectively in the amplified products of different primer combinations. These bands can be used to identify genotypes of B. dactyloides. The dendrogram and principal component analysis revealed from these markers clustered the materials into two main groups. But materials from the same geographic origins were not classified into the same group.关键词
野牛草/遗传多样性/SRAPKey words
Buchloe dactyloides genetic diversity sequence-related amplified polymorphism (SRAP)分类
农业科技引用本文复制引用
周莹洁,王显国,张新全..野牛草种质基于SRAP标记的遗传多样性研究[J].草业科学,2011,28(11):1930-1935,6.基金项目
中国农业大学科研启动基金项目 ()
国家自然科学基金项目 ()