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野生马蹄金花粉生活力检测方法比较

刘帮龙 张晓慧 干友民 贺啸尘 莫利

草业科学2011,Vol.28Issue(11):1941-1944,4.
草业科学2011,Vol.28Issue(11):1941-1944,4.

野生马蹄金花粉生活力检测方法比较

Detection methods of pollen viability of wild Dichondra repens

刘帮龙 1张晓慧 1干友民 1贺啸尘 1莫利1

作者信息

  • 1. 四川农业大学草业科学系,四川雅安625014
  • 折叠

摘要

Abstract

The pollen viability of wild Dichondra repent" ods of I2-KI, 4, 4'-diaminobiphenyl-l-Naphthol, TTC comparison of the four methods indicated that the meth wild Dichondra repens pollen activity. 4,4'-diaminobi (SD200303 and GD200503) was detected by methand pollen germination in vitro, respectively. The ods of I2-KI and TTC were not suitable to evaluate phenyl-l-Naphthol method could detect the pollen viability, but the measured value was higher than that of pollen viability. The method of germination in vitro was the fittest and convenient, in which pollen tube grew well and pollen viability was stable. The optimum culture medium was 10% Sucrose+0.005% HBO3 +0. 015% [Ca(NO3)2 4H20] +0.001% MgSO4 7 H2 O+ 0. 005 % KNO3 + 0.5 % Agar.

关键词

野生马蹄金/花粉生活力/检测方法

Key words

wild Dichondra repens pollen viability detection method

分类

农业科技

引用本文复制引用

刘帮龙,张晓慧,干友民,贺啸尘,莫利..野生马蹄金花粉生活力检测方法比较[J].草业科学,2011,28(11):1941-1944,4.

基金项目

四川省科技厅应用基础研究专项基金资金项目 ()

教育部春晖计划项目 ()

四川省科技厅项目 ()

草业科学

OA北大核心CSCDCSTPCD

1001-0629

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