草业科学2012，Vol.29Issue(2)：229-237,9.
早春短命植物绵果荠无菌苗组织培养相关因素研究
Influence factors for obtaining aseptic seedlings of early spring ephemeral Lachnoloma lehmannii in tissue culture
摘要
Abstract
In order to establish a system of aseptic seedling of Lachnoloma lehmannii in tissue culture, u- sing seeds as material, different disinfection methods of seeds were compared. The influence factors of seed germination and seedling growth were investigated. The results indicated that 1） a suitable method for seed sterilization was that seeds were washed under running tap water for 1--2 rain, soaked in 70% ethanol for 1 min and disinfected with 0.1% HgCl2 solution for 1.5 min. 2） Illumination was beneficial for seed germination, but different parts of seedlings had different responses to the lights. 3） Seed germina tion depend on a low temperature, while seedling growth required a certain high temperature. 4） Some hormones promote the seed germination, but different parts of seedlings had different reaction to the hor- mones. Therefore, the suitable culture system for seed germination and seedling growth of L. lehmannii was that seeds were placed in MS medium without growth regulators after seed sterilization, and cultured under lights at temperature of 15 ℃/5 ℃ for 4--5 d. Plates with germinated seeds were then transferred to a place with same illumination and temperature of 25 ℃ for seedling growth. The seed germination rate in- creased from 16% （control） to 90% and the seedlings grew well.关键词
早春短命植物/绵果荠/组织培养/无菌苗Key words
early spring ephemeral plant/Lachnoloma lehmannii/tissue culture/aseptic seedling分类
农业科技引用本文复制引用
曼苏尔·那斯尔,谭敦炎,廖康..早春短命植物绵果荠无菌苗组织培养相关因素研究[J].草业科学,2012,29(2):229-237,9.基金项目
新疆维吾尔自治区高技术研究与发展计划项目 （）
