草业学报2011，Vol.20Issue(6)：245-253,9.
利用EST-SSR标记分析西南扁穗牛鞭草种质的遗传多样性
Genetic diversity of Hemarthria compressa germplasms from southwestern China based on EST-SSR markers
摘要
Abstract
Using EST- SSR with primers from cereal crops, the genetic diversity was assessed in 40 wild Hem-arthria compressa clones from southwestern China and three varieties. After amplification with 23 pairs of EST - SSR primers of Poaceae, 323 fragments were detected, of which 261 were polymorphic, accounting for 80. 4% of the total. The polymorphic information content was from 0. 354 to 0. 500. The genetic similarity coefficient of 43 accessions ranged from 0. 690 to 0. 913. Clustering analysis also revealed that the clustering a-mong tested materials was related to their geographic origins, ecological types and morphological characteristics. AMOVA (analysis of molecular variance) of five geographical groups of H. Compressa revealed that 95. 32% of total variance occurred within these groups and 4. 68% of total variance occurred between them. It is suggested that using EST-SSR primers from cereal crops, is feasible and effective for analyzing the genetic diversity of H. Compressa.关键词
扁穗牛鞭草/EST-SSR/遗传多样性Key words
Hemarthria compressa/ EST-SSR marker/ genetic diversity分类
农业科技引用本文复制引用
陈永霞,张新全,谢文刚,马啸,刘影..利用EST-SSR标记分析西南扁穗牛鞭草种质的遗传多样性[J].草业学报,2011,20(6):245-253,9.基金项目
国家科技支撑计划(2011BAD17B03)和科技部农转资金(2010GB2F000402)资助. （2011BAD17B03）
