草业学报2012，Vol.21Issue(1)：126-132,7.
假俭草抗旱变异体的筛选及其生理鉴定
Selection and physiological identification of somaclonal variants for increased drought resistance of centipedegrass
摘要
Abstract
Centipedegrass (Eremochloa ophiuroides) is an important warm-season turfgrass species, originated from China, but its drought resistance needs to be improved. In this paper selection and identification of soma-clonal variants of centipedegrass for increased drought resistance were investigated. Embryogenic calli were subject to a long-term subculture for 24 months for induction of somaclonal variation. Five individual variants with improved drought resistance were selected and identified. Their physiological responses to drought were further measured. Relative water content (RWC) , ion leakage, and malondialdehyde (MDA) content decreased after drought stress by withholding irrigation, but higher RWC and lower levels of ion leakage and MDA were in the drought-resistant variants than the control plants. There was no significant difference in proline content and superoxide dismutase (SOD) and catalase (CAT) activities between the variants and the wild type control plants prior to drought treatment. After withholding irrigation for 6 d, all plants showed increase in proline content and SOD and CAT activities, while lower content of proline and higher activities of SOD and CAT were in the variants than the control plants. It is suggested that the increased drought resistance in the five variants is associated with their higher levels of SOD and CAT activity under drought stress.关键词
假俭草/体细胞变异/抗旱性/生理响应Key words
centipedegrass/ somaclonal variation/ drought tolerance/ physiological responses分类
农业科技引用本文复制引用
刘明稀,卢少云,郭振飞..假俭草抗旱变异体的筛选及其生理鉴定[J].草业学报,2012,21(1):126-132,7.基金项目
广东省科技计划项目(2006B20201035)和湖南农业大学校青年基金(10QN30)资助. （2006B20201035）
