草业学报2012，Vol.21Issue(1)：133-140,8.
氮素形态对盐胁迫下菊芋幼苗PSⅡ光化学效率及抗氧化特性的影响
Effects of nitrogen form on the photochemical efficiency of PS Ⅱ and antioxidant characteristics of Jerusalem artichoke seedling under salt stress
摘要
Abstract
In order to study the effect of different nitrogen form on the physiology of Jerusalem artichoke seedling under the salt stress, a sand cultural study was carried out under the nitrogen nutrition (NH4 + /NO3- as 4/1, 1/1 and 1/4) and salt stress. 1) The treatment of low concentration of salt stress had a little effect on the inhibition of plant growth, but high concentration of salt stress could remarkably inhibit Jerusalem artichoke growth. Under the same salt concentrations, the dry weights, relative moisture content, plant height and leaf area of Jerusalem artichoke increased with the proportions of NO3--N augment. 2) With the increasing of salt concentration, PS Ⅱ system was significantly damaged, but the increasing of the proportions of NO3--N could reduce the damage degree of the PS II system and improve ΦPS Ⅱ , Fv, Fv/Fm, Fv/Fo, Fm/Fo and qP. Especially, nitrogen nutrition had the highest light use efficiency at the ratio of NH4 + /NO3- as 1/4. For example, under 100 mmol/L NaCl stress, compared with the treatment at the ratio of NH4 + /NO3- as 4/1, ΦPSⅡ , Fv, Fv/Fm, Fv/Fo, Fm/Fo and qP of the treatment at the ratio of NH4 + /NO3- as 1/4 increased by 1. 03, 1. 15, 1. 05, 1. 26, 1. 20 and 1. 05 times, respectively. 3)Too much salt supply could cause the imbalance of plant cell osmotic, however, under the same salt concentration, the increasing of the proportions of NO3--N could significantly promote soluble sugar and soluble protein production and the transfer to the roots, then maintained the balance cell osmotic effectively under salt stress. 4)Under the salt stress, the membrane lipid peroxidation usually occurred in plants cells, but the proportions of NO3--N augment could promote the SOD, POD, CAT and APX activity which had a function of eliminating reactive oxygen species, then alleviated membrane damage which the excessive accumulation of reactive oxygen species caused in cell. For example, under 100 mmol/L NaCl stress, compared with the treatment at the ratio of NH4 + /NO3-as 4/1, SOD, POD, CAT and APX activities of the treatment with NH4 + /NO3- as 1/4 were improved by 1.19, 1.08, 1.18 and 1.11 times, respectively. So with respect to NH4 + -N, the increase of the proportions of NO3--N was more conducive to increase the accumulation of dry matter and water content, improve the activity of intracellular protection enzyme, maintain the stability of PSⅡ system and cell osmotic balance, then enhanced the resistance of Jerusalem artichoke to salt stress.关键词
菊芋/氮素/盐胁迫/光能利用效率/膜脂过氧化Key words
Jerusalem artichoke (Helianthus tuberosus)/ nitrogen/ salt stress/ light use efficiency/ membrane lipid peroxidation分类
农业科技引用本文复制引用
王磊,隆小华,郝连香,刘兆普..氮素形态对盐胁迫下菊芋幼苗PSⅡ光化学效率及抗氧化特性的影响[J].草业学报,2012,21(1):133-140,8.基金项目
江苏省自主创新资金项目(CX112052),国家科技支撑项目(2011BAD13B09和2009BADA3B04-8),公益性行业(农业)科研专项经费项目(200903001-05),中央高校基本业务费项目(Y0201100249),教育部博士点基金(20100097120016)和江苏省科技支撑项目(BE2010305和BE2011368)资助. （CX112052）
