草业学报2012，Vol.21Issue(1)：170-175,6.
利用RAPD技术构建四倍体苜蓿遗传连锁图谱
The construction of genetic linkage frame map in tetraploid Medicago using RAPD markers
摘要
Abstract
Using random amplified polymorphic DNA (RAPD) molecular genetic markers analyze the F2 population of 94 plant individuals. The F2 segregating population derived from a self-pollinated F1 hybrid individual of the cross Medicago sativa ×Medicago falcata. The genetic analyses were performed by using maximum-likelihood equations and related computer programs. The genetic map comprises 74 markers, and contains 8 linkage groups covering 1 261. 5 cM, with an average distance of 24. 73 cM between markers. This genetic linkage map provides an entry point for the construction of saturated tetraploid alfalfa molecular genetic map and further development of alfalfa molecular genetic research.关键词
苜蓿/RAPD/连锁图谱Key words
Medicago/ RAPD/ linkage map分类
农业科技引用本文复制引用
刘曙娜,于林清,周延林,吉仁花,陈世茹,孙娟娟,么婷婷..利用RAPD技术构建四倍体苜蓿遗传连锁图谱[J].草业学报,2012,21(1):170-175,6.基金项目
中央公益性科研院所基本科研业务费专项资金(中国农业科学院草原研究所),农业科技转化项目(2011)(GB23260017)资助. （中国农业科学院草原研究所）
