草业学报2012，Vol.21Issue(1)：206-212,7.
农杆菌不同菌液浓度对柳枝稷种子萌发及幼苗生长发育的影响
Switchgrass seeds germination and seedling growth and development with different concentration of Agrobacterium tume f aciens
摘要
Abstract
With the switchgrass strains (Xiji No. 1, Xiji No. 2, Xiji No. 3) as the dominated factors, the concentration of Agrobacterium tumefaciens as the deputy factors, the effect of switchgrass seed germination and seedling physio-biochemical characteristics were studied, using A. tumefaciens-soaking method. The results showed that the interaction effect between the strains and the concentration was not significant; The effects of A. tumefaciens on switchgrass strains were different, and the Xiji No. 3 was more insensitive to A. tumefaciens than the other strains. With the concentration of A. tumefaciens increased, the switchgrass seed germination rate, the seedling plantingpercent and chlorophyll content showed downtrend, and the seedling POD activity, CAT activity and MDA content showed uptrend, and the seedling SOD activity initially increased but then decreased. To sum up, the most appropiate concentration of A. tumefaciens may be under 1. 2 OD600 in switch-grass seeds soaking.关键词
柳枝稷/农杆菌/菌液浓度/浸种Key words
switchgrass (Panicum virgatum) / Agrobacterium tumefaciens / concentration/ soaking seed分类
农业科技引用本文复制引用
徐开杰,燕明献,柴乖强,孙风丽,刘曙东,奚亚军..农杆菌不同菌液浓度对柳枝稷种子萌发及幼苗生长发育的影响[J].草业学报,2012,21(1):206-212,7.基金项目
国家自然科学基金(30871571),国家948计划项目(2011-G1-19)和西北农林科技大学基本科研业务费专项资金(CX200903)资助. （30871571）
