材料科学与工程学报2011，Vol.29Issue(5)：711-714,4.
荧光胺定量检测磁性微球表面氨基
Quantitative Detection Method of Surface Amino Groups on Magnetic Microspheres Using Fluorescamine
摘要
Abstract
Fluorescamine quantitative detection method for detecting the density of surface amino groups of magnetic microspheres was established and the reaction conditions were optimized via the investigation of the optical reaction properties between fluorescamine and amino groups.It is found that the modified aminosilane carrying amino groups will be completely dissociated from the surfaces of microspheres after 60h under alkaline conditions,which renders a stable fluorescent signal after a further reaction of 20min with fluorescamine.It is shown that the fluorescent signal is most stable with pH of 8.0 in the reaction system.The detection method of the density of amino groups on magnetic microspheres based on fluorescamine is simple and fast with a high accuracy.关键词
荧光胺/磁性微球/氨基定量检测Key words
fluorescamine/magnetic microspheres/amino group quantitative detection分类
化学化工引用本文复制引用
宁普文,徐宏,张吉喜..荧光胺定量检测磁性微球表面氨基[J].材料科学与工程学报,2011,29(5):711-714,4.基金项目
国家自然科学基金资助项目 （）
上海市科委资助项目 （）
