重庆大学学报：自然科学版2011，Vol.34Issue(7)：131-136,6.
蜂蜜中多种有机磷农药残留的液相色谱-串联质谱检测
Simultaneous determination of multiple organophosphorus pesticides residues in honey by liquid chromatography tandem mass spectrometry
摘要
Abstract
The trichlorfon,dichlorvos,and coumafos are commonly used as organophosphorus pesticides to control pests or natural enemies of bees,but they easily contaminate the honey.A simultaneous determination method for trichlorfon,dichlorvos and coumafos residues in honey is developed by high performance liquid chromatography tandem mass spectrometry（HPLC-MS/MS）.Samples are extracted with ethyl acetate and concentrated extracts,and then the residue is dissolved by methanol,and separated by HPLC,detected by electrospray ionization（ESI）-MS/MS with multiple reaction monitoring mode（MRM）.It is found that the linear ranges of the three organophosphate pesticides are 20~500 μg/L with correlation coefficients over 0.994,the detection limits（LODs） are 0.1 μg/kg and the limits of quantification（LOQs） are 4 μg/kg for all of the three pesticides.The average recoveries of the three pesticides in spiked honeies at concentration levels of 10~40 μg/kg range from 78.5% to 96.8%,and the relative standard deviations are in the range of 9.1%~13.1%.关键词
色谱分析/液相色谱串联质谱/有机磷农药/残留检测/蜂蜜Key words
chromatographic analysis/liquid chromatography-MS/MS/organophosphate pesticides/residue determination/honey分类
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王国民,李应国,戴华,谢文,张进忠,周启明,李正国..蜂蜜中多种有机磷农药残留的液相色谱-串联质谱检测[J].重庆大学学报：自然科学版,2011,34(7):131-136,6.基金项目
国家认证认可监督管理委员会资助项目 （）
