重庆理工大学学报：自然科学Issue(11)：34-38,5.
人CD9基因的克隆及原核蛋白表达
Cloning and Prokaryotic Expression of Human CD9 Gene
摘要
Abstract
CD9 protein encoded by CD9 gene is a member of the transmembrane 4 superfamily,also known as the tetraspanin family.This protein mediates signal transduction events that play a role in the regulation of cell development,activation,growth and motility.Here the complete open reading frame（ORF） of CD9 gene was cloned by RT-PCR and prokaryotic expression vector of PET30a（＋）/CD9 was constructed.The PET30a（＋）/CD9 protein was expressed and purified.The results showed that the length of the ORF of CD9 was 690bp and the molecular weight of the expressed PET30a（＋）/CD9 protein was 30KD.All the data indicate that the ORF of CD9 gene was cloned and the PET30a（＋）/CD9 protein was expressed successfully.关键词
CD9基因/克隆/原核表达Key words
CD9 gene/cloning/prokaryotic expression分类
医药卫生引用本文复制引用
郑小莉,罗波,张艺,刘佳佳..人CD9基因的克隆及原核蛋白表达[J].重庆理工大学学报：自然科学,2011,(11):34-38,5.基金项目
国家自然科学基金资助项目 （）
四川省卫生厅基金资助项目 （）
