重庆医学2012，Vol.41Issue(4)：355-356,359,361,封3,5.DOI:10.3969/j.issn.1671-8348.2012.04.016
脐静脉内皮细胞诱导脐血单个核细胞向树突状细胞的分化
Human umbilical blood mononuclear cells differentiated into dendritic cells by induction of umbilical vein endothelial cells
齐莹莹 1杜英 2李倩如 2陈惠萍 3徐虹 4周云 5董子明2
作者信息
- 1. 郑州市人民医院检验科,450000
- 2. 郑州大学基础医学院微生物学与免疫学教研室,450001
- 3. 郑州大学基础医学院病理生理学教研室,450001
- 4. 河南省肿瘤研究所,郑州,450000
- 5. 河南省人民医院肿瘤科,郑州,450000
- 折叠
摘要
Abstract
Objective To investigate the influence of human umbilical vein endothelial cells(HUVEC) on the differentiation of umbilical blood mononuclear cells (UBMCs) to dendritic cells(DCs) for establishing the more convenient, faster and more economic in vitro culture method of DCs. Methods HUVKC was digested and isolated by collagenase. After identification and in vitro culture and amplification, HUVKC were tiled and grown in the bottom of Transwell plate. UBMCs were inoculated to Transwell small indoor, and at the same time protein antigen formed by KC9706 cell freezing and thawing was added and cocultured with HU-VEC. UBMCs were closely contacted with HUVEC and migrated from Transwell upper chamber to the below chamber. The chamber cells were collected under Transwell plate. The dendritic cells related surface marker CD83 ,CD80 ,CD40, Cdla and MHC molecules were detected by the flow cytometry. The MTT assay was adopted to detect the specific target cell killing effect of cytotoxic T lymphocyte(CTL) inducted by tumor-oaded UBDC. Results After induction of HUVEC and loading the tumor cell antigen, DC CD80,CD40,CD83,Cdla and MHC-II molecules expression were increased, generated CTL could specifically kill esophagal cancer cells EC9706,its killing rate to EC9706 had no statistical difference compared with that of CTL activated by cytokine-induced DC (P>0. 05). Conclusion Using Transwell chamber primary HUVEC for inducing the differentiation of UBMC to DCs is a feasible experimental method.关键词
树突状细胞/脐静脉内皮细胞/脐血单个核细胞Key words
dendritic cell/umbilical vein endothelial cells/umbilical blood mononuclear cells引用本文复制引用
齐莹莹,杜英,李倩如,陈惠萍,徐虹,周云,董子明..脐静脉内皮细胞诱导脐血单个核细胞向树突状细胞的分化[J].重庆医学,2012,41(4):355-356,359,361,封3,5.
