重庆医学2012，Vol.41Issue(5)：432-434,封2,4.DOI:10.3969/j.issn.1671-8348.2012.05.006
食管癌组织中存活蛋白的异常表达
Abnormal expression of survivin in human esophageal cancer tissue
摘要
Abstract
Objective To observe the correlation between survivin and the esophageal cancer by comparing the expression level of survivin in the cancer tissue and the adjacent tissue. Methods Recombinant plasmids pTA/survivin used as the standard plas-mids in quantitative real-time PCR was constructed. Quantitative real-time PCR and immunohistochemistry were used to evaluate the endogenous expression level of survivin mRNA and protein in 33 cases of esophageal cancer tissues (experimental group) confirmed by pathology and their corresponding adjacent tissues (control group). And the relationship between the expression level of survivin and the clinicopathologic parameters of esophageal cancer was also analyzed. Results FQ-PCR results showed that the positive expresstion rate of survivin in the experimental group was 81. 82% (27/33) , the control group was 39. 4% (14/33) , there was significant difference between the two groups when analyzed by the statistical software(P＜0. 05). The immunohistochemical results in the two groups were consistent with the FQ-PCR, but the positive cells of the control group were mostly inflammatory cells not the epithelial cells. There was no significant correlation between survivin expression and age, sex and tumor differentiation grade(PX). 05) ,but had significant relationship with tumor stage and lymph node metastases(P＜0. 05). Conclusion The expression of survivin in esophageal cancer tissue may be associated with tumorigenesis,invasion and lymph node metastasis.关键词
食管肿瘤/存活蛋白/聚合酶链反应Key words
esophageal neoplasms/survivin/polymerase chain reaction引用本文复制引用
蔡燕,陈先卓,彭乙华,唐中,刘青松,蒋红,杨明辉..食管癌组织中存活蛋白的异常表达[J].重庆医学,2012,41(5):432-434,封2,4.基金项目
四川省卫生厅科研基金资助项目(030016). （030016）
