重庆医学2012，Vol.41Issue(5)：453-454,457,封2,4.DOI:10.3969/j.issn.1671-8348.2012.05.014
利用绿色荧光蛋白进行microRNAs靶点分析的研究
Study of utilizing green fluorescent protein to analyze microRNAs targets
摘要
Abstract
Objective To investage the interaction between microRNA and its target using enhanced green fluorecence protein (EGFP) as report gene. Methods MiR-122a target gene hepatitis C virus 5 untranslated region (UTR) was inserted into pEGFP-C1 to construct plasmid pEGFP-UTR. pEGFP-UTR and miR-122a were cotransfected into HEK293 cell line. At 48h after transfec-tion,EGFP expression was detected by fluorecence microscope as well as Western blot. Meanwhile, the sensitivity of EGFP as report gene was evaluated by luciferase report gene. Results miR-122a could inhibit EGFP expression loading HCV UTR at its 3' untranslated region. And the sensitivity of EGFP was close to luciferase. Conclusion EGFP can be used as report gene to reflect microRNAs and target interaction clearly and directly,and the feature of EGFP make it a good material for screening microRNAs and its unkonwn target.关键词
微RNAs/靶点/增强绿色荧光蛋白/报告基因Key words
microRNA/target/enhanced green fluorecence protein/report gene引用本文复制引用
陈可,刘湘..利用绿色荧光蛋白进行microRNAs靶点分析的研究[J].重庆医学,2012,41(5):453-454,457,封2,4.基金项目
国家自然科学基金资助项目(30800971). （30800971）
