重庆医学2012，Vol.41Issue(6)：521-523,526,4.DOI:10.3969/j.issn.1671-8348.2012.06.001
基因沉默Delta N p63对膀胱肿瘤生长抑制和CDK4的影响
Inhibitory effect of tumor growth and CKD4 expressing by silencing Delta N p63 gene on human transitional cell carcinoma of bladder
摘要
Abstract
Objective To explore the inhibitory effect of tumor growth and ckdA expressing by silencing Delta N p63 gene with short hairpin RNA on human transitional cell carcinoma of the bladder(TCCB) in xenograft nude mice through RNAi technique. Methods Human TCCB 5637 cells were transplanted into nude mice to establish xenograft tumors, Delta N p63 specific shRNA expression plasmid was transfected into the tumors, the tumor volume was observed, tumor morphology was observed with HK staining. The inhibitory effect of ckd4 gene was examined with semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) , ckd4 protein was examined by the immunohistochemical staining assay and Western blot. Results Delta N p63 specific shRNA expression plasmid was transfected into the tumors. After 8 weeks, the tumor volume was significantly smaller in the treated group than in the control group and the normal saline group(P<0. 01) ,the inhibition rate was 74. 62% in the treated group. In the treated group the necrosis and decrease of karyokinesis were found under light microscope, inflammatory cells were also found, there was no such change in the control group and the normal saline group. Delta N p63-shRNA directly down-regulated the expression of ckd4 protein and mRNA. Conclusion The Delta N p63-shRNA expression plasmid can significantly inhibit the growth of TCCB in nude mice and also can inhibit the cellular proliferation of TCCB through inhibit ckd4.关键词
RNA干扰/细胞周期蛋白依赖激酶4/Delta N p63基因Key words
RNA interference/cyclin-dependent kinase 4/Delta N p63 gene引用本文复制引用
何云锋,吴小候,唐伟,王德林,尹志康,蒲军,陈刚..基因沉默Delta N p63对膀胱肿瘤生长抑制和CDK4的影响[J].重庆医学,2012,41(6):521-523,526,4.基金项目
重庆市卫生局科研基金资助项目(2011-2-103). （2011-2-103）
