摘要
Abstract
Objective To study the effects of hydrogen rich saline on endothelial reactive oxygen species(ROS) induction and apoptosis induced by high glucose. Methods Cells were divided into three groups,including control group,high glucose group,and H2 group (high glucose + hydrogen rich saline). The level of intracellular ROS was detected by FACS. The levels of super oxide dismutase(SOI)) ,and glutathione peroxidase(GSH Px) mRNA were detected by realtime PCR. The apoptosis of ceils was analyzed by cell death ELISA. Results The level of intracellular ROS was reduced in the H2 group,compared with the high glucose group (2.75±0. 37 vs. 6.35±1. 29,P<0.01). The levels of SOD and GSH-Px mRNA were higher in the H2 group than those in the highglucose group (SOD:0.74±0. 06 vs. 0.26±0.03,P<0.01;GSH Px:0. 68± 0.05 vs. 0. 31 ± 0. 04,P<0. 01). The apoptosis of cells in the H2 group was decreased,compared with the high glucose groups (0.38±0. 02 vs. 0.89±0. 05,P<0.01). Conclusion hydrogen rich saline can decrease the high glucose induced intracellular ROS induction, increase the antioxidase, and protect the high glucose induced apoptosis of endothelial cell.关键词
活性氧/氢气/葡萄糖/人脐静脉内皮细胞/凋亡Key words
reactive oxygen species / hydrogen / glucose / human umbilical vein endothelial cell / apoptosis
