重庆医学2012，Vol.41Issue(7)：630-632,636,封3,5.DOI:10.3969/j.issn.1671-8348.2012.07.002
持续小剂量化疗对人肺腺癌A549细胞裸鼠移植瘤VEGF-C及其受体Flt-4表达的影响
Influence of low-dose metronomic chemotherapy on expression of VEGF-C and its receptor Flt-4 in lung adenocarcinoma A549 cell xenografts in nude mice*
摘要
Abstract
Objective To explore the effects and mechanism of low-dose metronomic (LDM) chemotherapy on VEGF-C/Flt-4 axis in lung adenocarcinoma A54 9 cell xenografts in nude mice. Methods The human lung adenocarcinoma A54 9 cells were seeded in 18 nude mice to establish tumor-bearing animal models. They were divided into maximum tolerated dose (MTD) group (CTX 100 mg ? Kg-1 intraperitoneal injection every other day,3 consecutive times;n =6), LDM group (CTX 20 mg ? Kg-1 ? D-1 intraperito-neal injection,n= 6) and control group (0. 1 mL. Saline intraperitoneal injection once daily,n = 6) according to a random number table. The nude mice were sacrificed by neck luxation after 21 days of treatment, and subcutaneous tumor tissue were subjected to streptavidin-perosidase(SP) immunohistochemistry and reverse transcriptase-polymerase chain reaction (RT-PCR) for VEGF-C, Flt-4 protein and mRNA expression detection, respectively. Results VEGF-C,Elt-4 protein and mRNA expression level in tumor tissue of nude mice in LDM group were significantly lower than those in MTD group and control group (P<0. 05). Conclusion LDM chemotherapy may reduce lymphatic metastasis of lung adenocarcinoma A54 9 cell xenografts in nude mice through decreasing VEGF-C,Flt-4 expression in tumor tissue.关键词
血管内皮生长因子C/血管内皮生长因子受体3/肺肿瘤/淋巴管生成/小鼠,裸Key words
vascular endothelial growth factor C/vascular endothelial growth factor receptor-3/ lung neoplasms/ lymphangioge-nesis/ mice, nude引用本文复制引用
张明川,梅同华,厉明..持续小剂量化疗对人肺腺癌A549细胞裸鼠移植瘤VEGF-C及其受体Flt-4表达的影响[J].重庆医学,2012,41(7):630-632,636,封3,5.基金项目
重庆市医疗特色专科建设基金资助项目(200453). （200453）
