重庆医学2012，Vol.41Issue(7)：637-639,642,4.DOI:10.3969/j.issn.1671-8348.2012.07.004
姜黄素联合多烯紫杉醇诱导PC-3细胞凋亡的实验研究
Experimental study on induction of apoptosis in PC-3 cells by curcumin combined with docetaxel*
摘要
Abstract
Objective To investigate the effects of curcumin combined with docetaxel on apoptosis of human prostate cancer PC-3 cells and its mechanism. Methods Cultured PC-3 cells were divided into control group (only medium added) , curcumin group (treatment with 5. 0 μmol/L curcumin) ,docetaxel group (treatment with 2. 5 nmol/L docetaxel) and combination group (combination treatment of 5. 0 jumol/L curcumin and 2. 5 nmol/L docetaxel) ,and were treated for 24 hours. Flow cytometry was employed to analyze the apoptotic rate of PC-3 cells,reverse transcriptase-polymerase chain reaction(RT-PCR) and Western blot were used to detect the mRNA and protein expression of Bcl-2 and Bax, respectively. Results Both curcumin and docetaxel could induce apoptosis in PC-3 cells,the apoptotic rate of cells in combination group was higher than that in monotreatment groups(P<0. 05). RT-PCR and Western blot demonstrated that both curcumin and docetaxel down-regulated mRNA and protein expression of Bcl-2 and up-regulated mRNA and protein expression of Bax,and changes of Bax and Bcl-2 expression in combination group were more significant. Conclusion Curcumin and docetaxel can synergistically induce apoptosis in PC-3 cells via down-regulating Bcl-2 and up-regulating Bax.关键词
细胞凋亡/姜黄素/多烯紫杉醇/bcl-2/baxKey words
apoptosis/ curcumin/ docetaxel/ bcl-2/ bax引用本文复制引用
马强,王德林,赵修民,杨军..姜黄素联合多烯紫杉醇诱导PC-3细胞凋亡的实验研究[J].重庆医学,2012,41(7):637-639,642,4.基金项目
国家自然科学基金资助项目(30972999) （30972999）
重庆市自然科学基金资助项目(CSTC2005BB5033). （CSTC2005BB5033）
