重庆医学2012，Vol.41Issue(7)：643-645,3.DOI:10.3969/j.issn.1671-8348.2012.07.006
系统性红斑狼疮患者静脉血BCMA和CD138+浆细胞的研究
A study of BCMA and CD138+ plasmacytoid cells in peripheral blood of patients with systemic lupus erythematosus*
摘要
Abstract
Objective To study the differences of B cell maturation antigen(BCMA) expression and the amount of CD138+ plasmacytoid cells in venous blood between patients with systemic lupus erythematosus(SLE) and healthy people. Methods 40 blood samples of patients with SLE served as SLE group and 30 blood samples of healthy people served as control group. Fluorescent quantitative real time-polymerase chain reaction and enzyme-linked immunosorbent assay(ELISA) were used to detect the expression of BCMA. Flow cytometry was adopted to assay the amount of CD138+ plasmacytoid cells in venous blood. Results The mRNA level and protein content of BCMA and the amount of CD138+ plasmacytoid cells in venous blood of patients in SLE group were significantly higher than those in control group(P<0. 05). Conclusion The increased amount of plasmacytoid cells in patients with SLE may be related to the high expression of BCMA.关键词
B淋巴细胞/浆细胞/红斑狼疮,系统性/聚合酶链反应/酶联免疫吸附测定Key words
B-lymphocytes/ plasma cells/lupus erythematosus,systemic/polymerase chain reaction/ enzyme-linked immunosor-bent assay引用本文复制引用
牛晓昶,曾照芳,贾佳,李艳林,夏季,黄恒柳,邓少丽..系统性红斑狼疮患者静脉血BCMA和CD138+浆细胞的研究[J].重庆医学,2012,41(7):643-645,3.基金项目
国家自然科学基金资助项目(81101199,30972728) （81101199,30972728）
重庆市自然科学基金资助项目(2007BB5076). （2007BB5076）
