重庆医学2012，Vol.41Issue(7)：679-682,4.DOI:10.3969/j.issn.1671-8348.2012.07.020
康肤霜对糖尿病大鼠烫伤创面愈合的作用
Effect of Kangfu cream on the scald wound healing of rats with diabetes mellitus*
摘要
Abstract
Objective To observe the effects of Kangfu cream on deep Ⅱ degree burn wound healing of rats with diabetes melli-tus. Methods Tail-vein injection of alloxan in 24 Wister rats were performed to establish diabetic rat models. Automatic time control and pressure regulation scald apparatus was employed to make 4 surfaces of deep Ⅱ degree burn wound on the back of rats. The wound surfaces were randomly divided into Kangfu cream group, rhEGF group, povidone-iodine group and physiological saline group in which rats were treated with Kangfu cream, rhEGF, povidone-iodine and physiological saline, respectively, until the wound tissue samples taken or the wound healing. The wound healing rates and the healing periods were observed. The samples of wound surface tissue of rats in each group were taken on the 7th,14th and 21th day after treatment. Hematoxylin-eosin (HE) staining was used to detect the pathological changes of wound tissue by optical microscope. Streptavidin biotin-peroxidase complex(SABC) im-munohistochemistry was adopted to detect the expression of proliferating cell nuclear antigen(PCNA) ,and the PCNA positive cells were measured by labeling index method to evaluate the tissue repair in wound surface. Results Both wound healing period and healing rates of rat in Kangfu cream group were superior to those in the other 3 groups(P<0. 05). Pathological examination demonstrated that Kangfu cream could significantly reduce the early inflammatory response in wound surfaces,promote the wound re-epi-thelialization and the differentiation of each epidermal layer. The expression level of PCNA in wound surface tissue was markedly higher than those in the other 3 groups(P<0. 05). Conclusion Kangfu cream can promote the cell proliferation in wound surface of rats with diabetes mellitus and facilitate the wound healing.关键词
糖尿病,实验性/伤口愈合/增殖细胞核抗原/大鼠Key words
diabetes mellitus,experimental/wound healing/ proliferating cell nuclear antigen/rats引用本文复制引用
龚震宇,童亚林,万友华,杨润生,苏晓玲..康肤霜对糖尿病大鼠烫伤创面愈合的作用[J].重庆医学,2012,41(7):679-682,4.基金项目
广西自然科学基金资助项目(2060203-255). （2060203-255）
