重庆医学2012，Vol.41Issue(7)：683-685,3.DOI:10.3969/j.issn.1671-8348.2012.07.021
腺嘌呤致大鼠睾丸损伤中标志酶的变化及淫羊藿总黄酮的干预作用
Alteration of marker enzymes in adenine-induced impaired testis and the interference of total flavonoids of herba epimedii in rats
摘要
Abstract
Objective To study the interference effect of total flavonoids of herba epimedii(TFE) on marker enzymes in adenine-induced impaired testis in rats. Methods 70 Sprague Dawley male rats were randomly divided into 5 groups of 14 rats each; normal control group(intravenous infusion of distilled water) ,model groupCgastric perfusion of distilled water) ,TFE groupCgastric perfu-sion of TFE 100 mg/kg) , Vitamin E groupCgastric perfusion of vitamin E 10 mg/kg) and methyl testosterone group (gastric perfusion of methyl-testosterone 5 mg/kg) ,Except rats in normal group,those in the rest groups were subjected to gastric perfusion of adenine 150 mg/kg for 14 days to establish the rat models of testicular lesion. Rats in each group were accepted corresponding treatment from the 15th day,and then sacrificed after 30 days. The mass of testis, epididymis, seminal vesicles and rats were weighted,and the organ indexes were calculated. Serum testosterone,follicle-stimulating hormone(FSH) ,luteinizing hormone (LH) and testicular lactate dehydrogenase (LDH) , acid phosphatase ( ACP) , gamma-glutamyl transpeptidase(γ-GT),superoxide dismutase (SOD) ,glutathione peroxidase (GSH-Px) activity and malondialdehyde (MDA) content were detected. Results Compared with the normal control group,the gonadal organ indexes, serum testosterone value and testicular LDH,ACP,γ-GT activity decreased, while MDA content increased in model group,with significantly difference(P<0. 05). The rats organ indexes, serum testosterone levels and specific enzyme activity were increased in TFE group when compared to those in model group(P<0. 05). Conclusion TFE may improve the activity of marker enzymes in adenine-induced impaired testis through promoting the testosterone release and anti-oxidation.关键词
黄酮类/淫羊藿属/腺嘌呤/睾丸/大鼠Key words
flavones/ epimedium/ adenine/ testis/ rats引用本文复制引用
张作涛,谢高宇,陈凯,齐敏友,刘浩然..腺嘌呤致大鼠睾丸损伤中标志酶的变化及淫羊藿总黄酮的干预作用 [J].重庆医学,2012,41(7):683-685,3.基金项目
贵州省科技厅资助项目(黔科合中药[2011]LKZ7032号),浙江省教育厅科研重点项目(Z201121669). （黔科合中药[2011]LKZ7032号）
