重庆医学2012，Vol.41Issue(7)：686-688,3.DOI:10.3969/j.issn.1671-8348.2012.07.022
重负压封闭引流对兔皮肤创面炎症细胞趋化和炎症因子分泌的影响
Effects of vacuum sealing drainage on chemotaxis of inflammatory cells and secretion of inflammatory cytokines in skin wound of rabbits
杨帆 1胡耑 1白祥军 1李仁杰 1张锟 1薛晨晨 1曹圆1
作者信息
- 1. 华中科技大学同济医学院附属同济医院创伤外科,湖北武汉,430030
- 折叠
摘要
Abstract
Objective To observe the effects of vacuum sealing drainage(VSD) on inflammatory cells chemotaxis and inflammatory cytokines secretion in wound tissue of rabbits. Methods 12 rabbit skin wound models were established and randomly divided into conventional group (n= 6, disinfecting without special treatment, only routine dressing and bandage daily) and VSD group[n = 6 , disinfecting routinely and continuous suction of -125 mmHg ( -16. 6 kPa) after membrane affixed to the wound]. Wound tissue were collected at different time points and subjected to observation under optical microscopy after hematoxylin-eosin staining. Counting of macrophages, polymorphonuclear neutrophils (PMN) and lymphocyte were performed. Enzyme-linked immunosorbent assay(ELISA) was adopted to detect the contents of tumor necrosis factor-α(TNF-α) and interleukin-6 (IL-6) in wound tissue. Results Compared with VSD group and conventional group before modeling, there were no significant differences in macrophages, PMN and lymphocyte counting in wound tissue(PX). 05). Amounts of macrophages and PMN in wound tissue in two groups were both increased after modeling. Compared to conventional group, the amount of macrophages in wound tissue in VSD group was markedly increased(P<0. 05) , while those of PMN and lymphocytes were obviously decreased(P<0. 01). Contents of TNF-α and IL-6 in wound tissue in two groups were both increased after modeling and declined after achieving the peak value at the time point of 12 h. Contents of TNF-α and IL-6 in wound tissue in VSD group were notably lower than those of conventional group (P< 0. 05). Conclusion VSD reduces and localizes the inflammatory response through regulation chemotaxis of macrophages,PMN and lymphocytes as well as secretion of cellular TNF-α and IL-6.关键词
炎症趋化因子类/巨噬细胞/中性白细胞/淋巴细胞/负压封闭引流Key words
chemokines/macrophages/neutrophils/lymphocytes/vacuum sealing drainage引用本文复制引用
杨帆,胡耑,白祥军,李仁杰,张锟,薛晨晨,曹圆..重负压封闭引流对兔皮肤创面炎症细胞趋化和炎症因子分泌的影响[J].重庆医学,2012,41(7):686-688,3.
