重庆医学2012，Vol.41Issue(8)：769-771,封2,封3,5.DOI:10.3969/j.issn.1671-8348.2012.08.016
正常人角质形成细胞和黑素细胞体外共培养体系的建立
Establishment of coculture system of normal human epidermal keratinocytes and melanocytes in vitro
摘要
Abstract
Objective To establish coculture system of direct cell-to-cell contact between normal human epidermal keratinocytes and melanocytes in vitro. Methods Keratinocytes and melanocytes were cultured respectively,and then the coculture model of direct cell-to-cell contact between keratinocytes and melanocytes was established in vilro. Methyl thiazolyl tetrazolium(MTT) assay, UV spectrophotometry,levoDopa(L-Dopa) staining, immunohistochemical staining and transmission electron microscopy observation were employed to biologically identify the melanocytes and keratinocytes in the coculture system. Results Keratinocytes were round and grew in "cobblestone" pattern while melanocytes in bipolar or multi-level dendritic growth pattern when they were cultured separately. The 2 kinds of cells proliferated rapidly when they cocultured in a certain proportion. Melanocytes with 3 to 5 den-drites grew in crumb pattern with dozens of keratinocyte,similar to the structure of melanin unit. Melanocytes and keratinocytes in the coculture system exhibited normal biological functions. Conclusion Coculture system of direct cell-to-cell contact between normal human epidermal keratinocytes and melanocytes provides a model closer to physiological condition for melanocytes study in vilro.关键词
黑素细胞/角质形成细胞/共同培养技术/生物学鉴定法Key words
melanocytes/keratinocytes/coculture techniques/biological assay引用本文复制引用
李海东,王鹰..正常人角质形成细胞和黑素细胞体外共培养体系的建立[J].重庆医学,2012,41(8):769-771,封2,封3,5.基金项目
重庆市自然科学基金资助项目(CSTC2009BB5313). （CSTC2009BB5313）
