重庆医学2012，Vol.41Issue(8)：772-774,778,4.DOI:10.3969/j.issn.1671-8348.2012.08.017
羧甲司坦对慢性气道炎症大鼠肺组织中PI3K/AKT和γ-GCS表达的影响
Effects of carbocisteine on PI3K/AKT and γ-GCS expression in lung tissue of rats with chronic airway inflammation
李国吾 1夏熙郑 1刘待见1
作者信息
- 1. 郑州大学第二附属医院呼吸内科,郑州,450014
- 折叠
摘要
Abstract
Objective To observe effects of carbocisteine on phosphatidylinositol-3-kinase(PI3K)/protein kinase B(AKT) and y-glutamylcysteine synthetase (γ-GCS) expression in lung tissue of rats with chronic airway inflammation. Methods 30 healthy male SD rats were randomly divided into control group, model group and carbocisteine treatment group. Intratracheal instillation of lipopolysaccharide and cigarette smoking were resorted to establish rat models of chronic airway inflammation in model group and carbocisteine treatment group. Rats in carbocisteine treatment group were subjected to gastric irrigation with carbocisteine (500 mg/kg) 30 minutes before smoking from day 17 to day 30. Cells in bronchoalveolar lavage fluid (BALF) of rats in each group were counted,pathological changes of lung tissue were observed,immunohistochemical detection was adopted to detect the expression levels of y-GCS and phosphorylated AKT(p-AKT) and reverse transcriptase-polymerase chain reaction (RT-PCR) was employed to detect the expression of PI3K mRNA and γ-GCS mRNA. Results Compared with model group, inflammatory infiltration in bronchial and lung tissue and neutrophils in BALF of rats in carbocisteine treatment group were significantly lessened (P< 0. 05) ,their p-AKT protein,γ-GCS protein, PI3K mRNA and γ-GCS mRNA expression were markedly decreased(P<0. 05) ,and the contents of glutathione(GSH) ,reactive oxygen species(ROS) were obviously reduced, while that of total antioxidative capability significantly increased(P<0. 05). Conclusion Carbocisteine probably reduces the inflammation and oxidative stress in lung of rats suffering from passive smoking through improving the expression of y-GCS protein via PI3K/AKT pathway.关键词
磷脂酰肌醇3-激酶/蛋白激酶类/谷胱甘肽/羧甲司坦/气道炎症Key words
phosphatidylinositol 3-kinase/protein kinases/glutathione/carbocisteine/airway inflammation引用本文复制引用
李国吾,夏熙郑,刘待见..羧甲司坦对慢性气道炎症大鼠肺组织中PI3K/AKT和γ-GCS表达的影响[J].重庆医学,2012,41(8):772-774,778,4.
