重庆医学2012，Vol.41Issue(9)：881-883,887,4.DOI:10.3969/j.issn.1671-8348.2012.09.018
hfgl2凝血酶原酶基因5'端调控序列的克隆及生物信息学分析
Coloning of 5′ regulatory sequence of hfgl2 prothrombinase gene and its bioinformatic analysis*
摘要
Abstract
Objective To amplify 5' regulatory sequence of human fibrinogen-like protein 2(hfgl2) prothrombinase gene, construct expression vectors with luciferase reporter gene and predict the possible regulation regions and cis-acting elements through bioinformatic analysis of 5' flanking promoter sequences of hfgl2 gene. Methods Polymerase chain reaction (PCR) was used to amplified the 5' regulatory sequence of hfgl2 prothrombinase gene from genomic DNA of human peripheral blood mononuclear cells. PCR products was digested and cloned into pGL3-Basic vector. The recombination plasmid was subjected to enzyme digestion and sequencing, and the online analysis software was employed to predict the transcription factor binding sites and the promoter sequences in 5' flanking regulatory sequences. Results A length of 1 567 bp 5' flanking sequence of hfgl2 prothrombinase gene was amplified. Knzyme digestion and sequencing confirmed that the constructed plasmid pGL3-hfgl2 insert fragment was correct,and the analysis demonstrated that the gene sequence including a total of 138 cis-acting elements which were in 31 kinds. Conclusion Construction of luciferase reporter gene vector containing regulatory sequences of proximal promoter of hfgl2 gene lays a foundation for the study of transcription regulation of hfgl2 gene.关键词
转录启动子/质粒/转录调控/纤维蛋白原样蛋白/顺式作用元件Key words
transcription initiation site/plasmids/transcription regulation/human fibrinogen like protein/cis-acting elements引用本文复制引用
李学军,张灏,李永敢,孙碧红..hfgl2凝血酶原酶基因5'端调控序列的克隆及生物信息学分析[J].重庆医学,2012,41(9):881-883,887,4.基金项目
广西壮族自治区自然科学基金资助项目(桂科自0447025). （桂科自0447025）
