摘要
Abstract
To optimize the solid-phase extraction method of isolation, purification and determination of intracellular Microcystin-RR, -LR （ MC-RR,-LR ） , the sample was a black big head carp which enriched Microcystins. A method for trace level analysis of Microcystins in aquatic product using SPE and HPLC was developed, and the aquatic product was detected by diode array detection （ LC-DAD ） at 238 nm. During extraction, samples which enriched Microcystins were treated with different solvent （ 5 % acetic acid, 75 % methanol and mixed solvents 1-butanol,methanol,and water （ 1 ∶ 4 ∶ 15 ） ,eluted with 80 % methanol. Further, gradient elution was employed to separate microcystins in high performance liquid chromatography （ HPLC ） , the mobile phase consisted of 55 % methanol with small amount of trifluoroacetate acid （ TFA ） .The recoveries of MC-RR and MC-LR were 84.1 % and 89.9 % ,respectively. And the linear range is from 0.25 to 5g/L.The method is simple, sensitive, stable and suitable for the determination of the two most common Microcystins -LR,-RR.关键词
微囊藻毒素/高效液相色谱/固相萃取Key words
microcystins/high-performance liquid chromatograph/solid phase extraction分类
资源环境
