大豆科学2011，Vol.30Issue(6)：889-894,6.
过表达GmNHX1基因提高大豆根系的耐盐性
Enhancing Salt Tolerance of Soybean Roots by Overexpression of GmNHX1
摘要
Abstract
Na+/H+ antiporter is responsible for the regulation of cytoplasmic pH,sodium concentration and cell volume for plants to cope with salt stress. To enhance the salt tolerance of soybean,the GmNHX1 cDNA that encodes a soybean Na+/H+ antiporter protein was driven by the Cauliflower Mosaic Virus 35S promoter and overexpressed in soybean roots via Agrobacteri-um rhizogenes mediated transformation. Hpt screening,GUS and RT-PCR analysis all confirmed that GmNHXl was successfully integrated into the genome of soybean hairy roots. Salt tolerance analysis showed that the growth in length and weight of the transgenic hairy roots on MS agar medium were significantly better than the non-transgenic control, when supplemented with 100,150 and 200 mmol-L-1 NaCl. Both the cotyledons and composite plants with transgenic hairy roots survived better than the non-transgenic control when subjected to salinity stress. These results support the possibility of using GmNHXl to improve salt tolerance in soybean.关键词
大豆/发根农杆菌:GmNHX1:根系/耐盐性Key words
Soybean/Agrobacterium rhkogenes/GmNHX1/Roots/Salt tolerance分类
农业科技引用本文复制引用
王敏娟,侯文胜,王庆钰,林汉明,韩天富..过表达GmNHX1基因提高大豆根系的耐盐性[J].大豆科学,2011,30(6):889-894,6.基金项目
国家大豆产业技术体系建设专项(CARS-04) （CARS-04）
国家转基因生物新品种培育重大专项(2008ZX08010-004) （2008ZX08010-004）
