动物学研究2011，Vol.32Issue(6)：641-646,6.DOI:10.3724/SP.J.1141.2011.06641
半滑舌鳎血红蛋白α1的基因克隆及其在短期低氧胁迫下的表达分析
Cloning of hemoglobin-α1 from half-smooth tongue sole (Cynoglossus semilaevis) and its expression under short-term hypoxia
摘要
Abstract
This study cloned the hemoglobin α1 from the marine teleost,the half-smooth tongue sole (Cynoglossus semilaevis),and then examined its expression under hypoxia exposure.The full-length of CsHb-tl (594 bp) cDNA contains an open reading frame encoding 144 amino acids.Sequence analysis shows that the predicted CsHb-α1 amino acids shares high identities with that of other species.Real-time PCR showed that CsHb-α1 was highly expressed in the heart,liver,spleen,kidney and blood.Five to 120 min esposure and long-term (36 h) exposure to hypoxia (1.0 mg/L)significantly increased CsHb-α1 mRNA expression in most tissues compared to those fish held in normoxic conditions (dissolved oxygen (DO):6.2 mg/L).These results suggested that the up-regulation of Hb-α1 is an important component for adaptation of half-smooth tongue sole to short-term hypoxia.关键词
半滑舌鳎/血红蛋白/基因克隆/低氧胁迫Key words
Cynoglossus semilaevis/ Hemoglobin/ Gene clone/ Hypoxia stress分类
生物科学引用本文复制引用
王资生,齐志涛,田敬云,仇明,赵卫红,王爱民,黄金田,郭锡杰..半滑舌鳎血红蛋白α1的基因克隆及其在短期低氧胁迫下的表达分析[J].动物学研究,2011,32(6):641-646,6.基金项目
This study was financially supported by Nature Science Foundation of Jiangsu Province (BK2011418),the Graduate Research and Innovation of Colleges and Universities in Jiangsu Province (CX09B_ 106Z) and Natural Science Foundation of the Jiangsu Higher Education Institutions of China (09KJD240004) （BK2011418）
