动物学研究2011，Vol.32Issue(6)：651-656,6.DOI:10.3724/SP.J.1141.2011.06651
不同染色方法揭示纤毛原生动物草履虫接合生殖过程的新细节
New details indicated by different stainings during conjugation of ciliated protozoa Paramecium
摘要
Abstract
During conjugation of Paramecium caudatum,nuclear events occur in a scheduled program.Morphological studies on nuclear behavior during conjugation of P.caudatum have been performed since the end of the 19th century.Here we report on new details concerning the conjugation of P.caudatum through the staining of conjugating cells with protargol,carbol fuchsin solution,Hoechst 33342 and immunofluorescence labeling with monoclonal antibody of anti-α tubulin.1) The crescent nucleus is a characteristic of the meiotic prophase of P.caudatum,has an unstained area.We stained this area with protargol,which was separated from the chromatin area and was not detected by the other stainings.2) In regards to the four meiotic products,it has long been considered that only one product enters the paroral cone region (PC) and survives after meiosis.However,our protargol and immunofluorescence labeling results indicated that PC entrance of the meiotic product happened before the completion of meiosis instead of after.3) In our previous study,protargol staining indicated the presence of a swollen structure around the central part of the "U" and "V" shaped spindles connecting the two types of prospective pronuclei.However,immunofluorescence labeling with anti-α tubulin antibodies gave a different image from protargol.All these observations form the basis for further studies of their molecular mechanisms.关键词
草履虫/接合生殖/新月核/进入口旁锥/核间连丝Key words
Paramecium/ Conjugation/ Crescent/ PC entrance/ Connecting spindles分类
生物科学引用本文复制引用
高欣,杨仙玉,朱嘉骏,袁进强,王逸雯,宋敏国..不同染色方法揭示纤毛原生动物草履虫接合生殖过程的新细节[J].动物学研究,2011,32(6):651-656,6.基金项目
国家自然基金项目(30670397 （）
31071181) （）
教育部留学回国人员科研启动基金项目([2007]1108) （[2007]1108）
浙江省教育厅一般项目(Y200804256) （Y200804256）
浙江农林大学科研发展基金项目(2292000030) （2292000030）
浙江省大学生科技创新活动计划(新苗人才计划)项目(2010R412003) （新苗人才计划）
浙江农林大学大学生科技创新活动项目(100203,100224) （100203,100224）
