动物学研究2012，Vol.33Issue(1)：7-13,7.DOI:10.3724/SP.J.1141.2012.01007
EV71可感染幼龄中缅树鼩
Experimental studies on infant Tupaia belangeri chineses with EV71 infection
摘要
Abstract
Tupaia belonged are small mammals with a squirrel-like appearance; they were formerly classified under the primates order despite the lack of derived features characteristic of primates. Given that T. Belangeri are easy to raise, cheap to maintain, and have a small body size, a high reproductive rate, and close affinity to primates, these animals would be used as an alternative to primates in biomedical research. Three-month old T. Belangeri chineses were infected with enterovirus 71 (EV71) via three different routes, namely, oral administration, nasal dripping, and tail intravenous injection, to study the infection in infant T. Belangeri and find a feasible scheme to make them an ideal animal model of EV71 in place of primates. Daily activities were regularly observed, body temperatures were measured, and blood tests were conducted. Blood and fecal samples were regularly collected. The infection was examined via the neutralizing antibody test, reverse transcription polymerase chain reaction (RT-PCR), Real-Time PCR, and pathological analysis. The temperature, as well as the white blood cell count and the number of lymphocytes, increased four days after infection. Virus loads were determined in all three groups, and the peak appeared on, before, or after the tenth day, respectively. Thus, oral administration proved to be the best route. The highest serum antibody titer obtained was 1:16. Acute paralysis with urinary retention manifested after about two weeks, and pathological changes were observed in the brain, heart, lung, spleen, kidney, and other tissues. In conclusion, T. Belangeri chineses can infected with EV71 via oral administration, nasal dripping, and tail intravenous injection. Therefore, T. Belangeri are potential EV71 animal models for further studies on the mechanism of pathogenesis or vaccine evaluation.关键词
手足口病/EV71/中缅树鼩/动物模型Key words
Hand-foot-mouth disease/ EV71 virus/ Tupaia belangeri chineses/ Animal model分类
生物科学引用本文复制引用
王文广,黄晓燕,徐娟,孙晓梅,代解杰,李琦涵..EV71可感染幼龄中缅树鼩[J].动物学研究,2012,33(1):7-13,7.基金项目
"十一五"国家科技支撑计划项目(2009BAI83B02 （）
2011BAI15B01) （）
云南省科技基础条件平台建设计划项目(2006PT07-2) （2006PT07-2）
国家"973"计划项目(2011CB504903) （2011CB504903）
国家自然科学基金(81171573) （81171573）
云南省应用基础研究计划面上项目(2011FB116) （2011FB116）
