动物营养学报2011，Vol.23Issue(12)：2183-2189,7.DOI:10.3969/j.issn.1006-267x.2011.12.021
抗菌肽对蛋鸡血清免疫指标及脾脏白细胞介素2mRNA表达量的影响
Effects of Antimicrobial Peptides on Serum Immune Indices and IL-2 mRNA Expression in Spleen of Laying Hens
摘要
Abstract
The objective of this experiment was to study the effects of different dietary levels of antimicrobial peptides (AMPs) on serum immune indices and IL-2 mRNA expression in spleen of laying hens. Four hundred and eighty 30-week-old Hisex hens were randomly divided into four groups with four replicates per group and thirty hens per replicate. Hens in control group (group I ) were fed a basal diet, and the others in experimental groups (groups II , III and IV) were fed the basal diet supplemented with 200, 400 and 600 mg/kg AMPs, respectively. The experiment lasted for four weeks. The results showed as follows; dietary AMPs in experimental group could increase spleen coefficient, the spleen coefficient in group III was significantly higher than that in control group (P <0.05). Compared with the control group, the content of serum immunoglobu-lin A in groups II , III and IV was increased by 7. 69% (P >0. 05) , 19.40% (P <0. 05) and 14. 38% (P > 0. 05) , respectively, and serum immunoglobulin G content was increased by 12.69% (P>0.05),21.24% ( P < 0.05) and 9.10%(P>0.05), respectively. However, there were no significant effects on the contents of serum immunoglobulin M, complement 3 and complement 4. The expression level of IL-2 mRNA in spleen in groups II and III was significantly higher than that in groups I and IV (P <0. 05) , but there was no significant difference between group I and group IV (P >0. 05). In conclusion, AMPs can enhance immune function of laying has, and adding 400 mg/kg AMPs to diets for laying hens has the optimum effect.关键词
抗菌肽/蛋鸡/免疫功能/IL-2 mRNAKey words
antimicrobial peptides/ laying hens/ immune function/ IL-2 mRNA分类
农业科技引用本文复制引用
吕尊周,袁肖笑,蔡兆伟,尹兆正..抗菌肽对蛋鸡血清免疫指标及脾脏白细胞介素2mRNA表达量的影响[J].动物营养学报,2011,23(12):2183-2189,7.基金项目
浙江省重大科技专项(2008C12075-2) （2008C12075-2）
