动物营养学报2011，Vol.23Issue(12)：2210-2216,7.DOI:10.3969/j.issn.1006-267x.2011.12.025
海南霉素对体外瘤胃微生物发酵和氮代谢的影响
Hainanmycin: Effects on Rumen Microbial Fermentation and Nitrogen Metabolism in Vitro
摘要
Abstract
The effects of hainanmycin on rumen microbial fermentation and ruminal nitrogen metabolism in vitro were investigated in this study. Four doses of hainanmycin were evaluated using a 24 h batch culture of rumen fluid in vitro, with a forage-to-concentrate diet ratio of 60:40 (18% crude protein, 30% neutral detergent fiber, and 22% acid detergent fiber). The groups were as follows; control group (CON) , 10 mg/L monensin group (MON, positive control group) , and the four doses of hainanmycin groups (H0. 1, H1. 0, H10.0 and H100. 0) , I. E. , 0.1, 1. 0, 10. 0 and 100. 0 mg/L hainanmycin. After 24 h, the pH was immediately measured. Samples were collected and analyzed for concentrations of peptide, amino acids, ammonia nitrogen (NH3-N) and volatile fatty acids (VFA) , activities of protease and deaminase, and DNA isolation. The results showed that H100. 0 decreased the total VFA concentration and increased the final pH (P <0. 05). H10. 0, H100. 0 and MON reduced the acetate-to-propionate ratio, as well as the concentrations of acetate and butyrate, but increased the propionate concentration compared with CON (P <0. 05). H0. 1 and H1.0 had no significant effects on rumen microbial fermentation (P > 0. 05). H10. 0, H100. 0 and MON increased protease activity and concentrations of amino acids and peptide (P <0. 05) , but decreased NH,-N concentration and deaminase activity (P <0. 05). H10. 0, HI00. 0 and MON also increased the relative population size (RPS) of Prevotella ruminicola and genus Prevotella, but reduced the RPS of protozoa (P<0.05). H10.0, H100.0 and MON had no significant effects on the RPS of Selenomonas ruminantium, Megasphaera elsdenii and Rumi-nobacter amylophilus (P > 0. 05) , but significantly decreased the RPS of Butyrivibrio fibrisolvens (P < 0. 05). In terms of improving rumen fermentation and increasing non-ammonia nitrogen flow, appropriate dose of hainanmycin is 10. 0 mg/L. Moreover, Hainanmycin exhibited an action similar to that of monensin on the mode of rumen fermentations. In addition, hainanmycin regulated nitrogen metabolism by changing the number of Prevotella and protozoa.关键词
海南霉素/剂量/瘤胃发酵/氮代谢Key words
hainanmycin/ dose/ rumen fermentation/ nitrogen metabolism分类
农业科技引用本文复制引用
王志博,张永根,辛杭书,刘薇,夏科..海南霉素对体外瘤胃微生物发酵和氮代谢的影响[J].动物营养学报,2011,23(12):2210-2216,7.基金项目
农业部现代农业(奶牛)产业技术体系(nycytx-02-02) （奶牛）
