南方医科大学学报2011，Vol.31Issue(12)：2012-2015,4.DOI:44-1627/R.20111213.1622.001
IL28B基因多态性与慢性乙型肝炎病人对干扰素应答的关系
Relationship between single nucleotide polymorphisms in IL28B gene and response to interferon treatment in chronic hepatitis B patients
摘要
Abstract
Objective To explore the relationship between the single nucleotide polymorphisms (SNPs) of rs12979860 and rs8099917 in IL28B gene and the response to interferon treatment in hepatitis B e antigen (HBeAg)-positive patients with chronic hepatitis B patients. Methods Peripheral blood samples were collected from 82 HBeAg-positive patients with chronic hepatitis B receiving interferon treatment, including 38 with favorable response to the treatment and 44 without response. IL28B gene was amplified from the chromosomal DNA, and rs8099917 SNP was genotyped based on PCR-RLFP and rsl2979860 SNP by sequencing. Results In the responsive patients, the distribution frequencies of TT and TG+GG genotypes and allele G in SNPrs8099917 were 81.6% (31/38), 18.4% (7/38), and 9.2% (7/ 76), as compared to the frequencies of 97.7% (43/44), 2.3% (1/44), and 1.1% (1/88) in nonresponsive patients, respectively. The frequencies showed significant differences between the responsive and nonresponsive patients (P= 0.014 for genotypes and P=0.025 for allele G). The distribution frequencies of CT genotypes and allele T in SNPrsl2979860 showed no differences between the responsive and nonresponsive patients (P0.05). Conclusion rs8099917 SNP is probably associated with the response to interferon treatment in HBeAg-positive patients with chronic hepatitis B, and Allele G may be predictive of the treatment success.关键词
IL28B基因/乙型肝炎病毒/干扰素治疗/单核苷酸多态性Key words
IL28B gene/ Hepatitis B virus/ Interferon treatment/ single nucleotide polymorphism分类
医药卫生引用本文复制引用
陈楚明,周福元,周元平,曾艳丽,李威,侯金林..IL28B基因多态性与慢性乙型肝炎病人对干扰素应答的关系[J].南方医科大学学报,2011,31(12):2012-2015,4.基金项目
国家自然科学基金(30771899) （30771899）
