南方医科大学学报2011，Vol.31Issue(12)：2016-2020,5.DOI:44-1627/R.20111209.1709.006
瑞芬太尼预处理对大鼠肝脏缺血再灌注损伤的影响及机制
Protective effect of remifentanil preconditioning against hepatic ischemia-reperfusion injury in rats: role of p38 mitogen-activated protein kinases
摘要
Abstract
Objective To assess the role of p38 mitogen-activated protein kinases (p38MAPK) in the protective effect of remifentanil preconditioning (RPC) on hepatic ischemia-reperfusion injury in rats. Methods Ninety-six male SD rats were randomly assigned into sham-operated group, ischemia-reperfusion group (I/R group), RPC group, and SB (an inhibitor of p38 MAPK) +RPC group. The rats were sacrificed at the end of reperfusion, and serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), tumor necrosis factor-α (TNF-α), and interleukin-1β (IL-1β) were measured. HE staining was used to observe the hepatic histopathological changes, and Western blotting was employed to examine p38MAPK and pp38MAPK protein expression. TUNEL staining was used to examine cell apoptosis in the liver tissues. Results Compared with sham-operated group, I/R group showed significantly increased serum levels of AST, ALT, TNF-a and IL-1β with obvious histopathological changes and cell apoptosis in the liver. RPC significantly decresed the elevated serum levels of AST, ALT, TNF-a and IL-1|3 and lessened hepatic histopathological changes, and caused reduced p38MAPK phosphorylation and hepatic cell apoptosis index. The protective effects of RPC were abolished by SB 203580 pretreatment. Conclusion RPC attenuates the production of inflammatory factors by activating p38MAPK signal pathway to improve hepatic ischemia-reperfusion injury, and these effects can be blocked by SB203580, a p38MAPK inhibitor.关键词
p38丝裂原活化蛋白激酶/瑞芬太尼/肝/缺血再灌注损伤/炎性因子Key words
p38 mitogen-activated protein kinases/ remifentanil/ liver/ ischemia-reperfusion injury/ ischemia precondition/inflammatory factors分类
医药卫生引用本文复制引用
赵鸽,陈正春,申新,陈亚丽,吕毅..瑞芬太尼预处理对大鼠肝脏缺血再灌注损伤的影响及机制[J].南方医科大学学报,2011,31(12):2016-2020,5.基金项目
陕西省自然科学基金(SJ08C213) （SJ08C213）
