福建农业学报2011，Vol.26Issue(6)：1027-1033,7.
柑橘黄龙病病原菌的鉴定及16S rDNA序列分析
Identification and 16S rDNA Sequence Analysis of Citrus Huanglongbing Pathogen
摘要
Abstract
Nested-PCR detection of Shunchang citrus Huanglongbing pathogen was carried out in this study. Phylogenetic tree based on rDNA sequencing was built to find out the evolutionary between different parts citrus HLB pathogens. Digesting the target DNA fragment of 16S rDNA with restriction enzyme Xbal was carried out to confirm the species of Shunchang Huanglongbing pathogen. The results showed that there were 7 positive detections in 12 selected samples, and the rate of positive detections was 58.33%, and four were dominant character, three were recessive character. The results showed that Shunchang citrus Huanglongbing pathogen belong to Candidatus Liberibacter asiaticus based on the 16S rDNA phylogenetic analysis and restriction enzyme Xbal digestion analysis. And 16S rDNA sequence gap analysis showed that the difference between Ca. L. Asiaticus and Ca. L. Africanus, Ca. L. Africanus and Ca. L. Americanus, Ca. L. Americanus and Ca. L. Asiaticus were 2.75%, 3.90%, 3.44%, respectively.关键词
柑橘黄龙病/鉴定/16S rDNA/系统进化分析Key words
Citrus Huanglongbing/ Identification/ 16S rDNA/ Phylogenetic analysis分类
生物科学引用本文复制引用
孙大光,郑雪芳,刘波,阮传清,夏育陆,段永平..柑橘黄龙病病原菌的鉴定及16S rDNA序列分析[J].福建农业学报,2011,26(6):1027-1033,7.基金项目
国家公益性行业(农业)科研专项(201003067-05) （农业）
中美合作项目——柑橘木虱及黄龙病野外研究(2010-1652-02)、抗虫柑橘品种研究(6618-22000-037-01S) （2010-1652-02）
福建省财政专项——福建省农业科学院科技创新团队建设基金(STIF-Y03) （STIF-Y03）
