福建畜牧兽医2011，Vol.33Issue(5)：1-4,4.
APES处理的载玻片在原位杂交技术中的应用
Glass Slides Treated by Improved APES Method were Used in in situ Hybridization
摘要
Abstract
Objective：To obtain a kind of cheap glass slides without RNase pollution,then could be used to in situ hybridization.Method：An improved APES（3-Aminopropyl triethoxysilane） treatment method was used to remove RNase pollution through baking glass slides at high temperature and diluting APES with acetone.These glass slides were used to stick the paraffin and frozen tissues,then the experimental effects of these glass slides were evaluated by in situ hybridization.Results and Conclusion：Both paraffin and frozen sections were well fit for in situ hybridization without any ecclasis of tissues,and specific RNA signals were detected.Our findings indicated that the effects of these APES-treated glass slides could be equal to the imported ones.Therefore,we concluded these APES-treated glass slides can absolutely be used to in situ hybridization,moreover,the experimental cost will be saved remarkably.关键词
载玻片/APES/石蜡切片/冰冻切片/原位杂交Key words
Glass slide APES Paraffin section Frozen section in situ hybridization分类
农业科技引用本文复制引用
滕鸿琦,施珏平,肖爱平,李小兵,张明凤..APES处理的载玻片在原位杂交技术中的应用[J].福建畜牧兽医,2011,33(5):1-4,4.基金项目
国家自然科学基金青年基金项目（31000643）、国家自然科学基金项目 （）
卫生部科学研究基金-福建省卫生教育联合攻关计划资助项目（WKJ2008-2-51）资助 （）
