辐射防护2011，Vol.31Issue(5)：306-311,6.
X射线致人外周血线粒体DNA 4977bp缺失的时效关系研究
Time-effect Relationship of Mitochondrial DNA 4977bp Deletion in Human Peripheral Blood Cell after X Ray Irradiation
摘要
Abstract
To investigate the time-effect of mitochondfial DNA 4977bp deletion in human peripheral blood cells exposed to X ray, human peripheral whole blood samples were collected from two healthy individuals, and exposed to X rays with dose from 0 to 10 Gy. The genomic DNAs were isolated from the whole-blood samples, and the levels of mtDNA 4977bp deletion and copy number of total mtDNA in the DNA samples were detected by Real-time PCR after irradiation at 2, 12, 24, 48, and 72 h, respectively. The results showed that the copy number of mtDNA 4977bp deletion and total mtDNA, and the rates of mtDNA 4977bp deletion increase with incubation time with dose at 5 Gy after irradiation. Moreover, they increased with dose from 0 to 10 Gy after irradiation at 24 h and 72 h, respectively. The results suggested that the levels of mtDNA 4977bp deletion and the copy number of total mtDNA in human peripheral blood cells exposed to X ray were accumulated with incubation time and dose increase, respectively.关键词
X射线/线粒体DNA/4977bp缺失/时效关系Key words
X Rays/Mitochondrial DNA/4977bp Deletion/Time-effect Relationship分类
医药卫生引用本文复制引用
王金合,王平,姜峰,韩林,王喜爱,吕玉民..X射线致人外周血线粒体DNA 4977bp缺失的时效关系研究[J].辐射防护,2011,31(5):306-311,6.基金项目
河南省医学科技攻关创新项目（2005077）. （）
